Challenge Test for Listeria monocytogenes. Requirements for testing laboratories

300,00 €

The Challenge Test is a key tool for validating the shelf life of food products, especially in ready-to-eat items. Its proper execution is critical for food safety and regulatory compliance. With the Challenge Test, it is possible to evaluate the ability of a food product to inhibit the growth of pathogenic microorganisms, such as Listeria monocytogenes, one of the most relevant pathogens in the food industry due to its ability to grow in diverse environments.

This course will provide you with the necessary foundations to:

· Design a solid Challenge Test plan in compliance with regulations.

· Select matrices, strains, inoculation, and storage conditions.

Modalidad

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Categoría: Scheduled Courses

Programación Streaming
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INTRODUCTION: LISTERIA MONOCYTOGENES
- Brief introduction to the microorganism Listeria monocytogenes and its pathogenic characteristics
- History of incidents and evolution
LEGISLATION ASSOCIATED WITH L. MONOCYTOGENES
- Regulation 2073/2005
- Regulation 2024/2895
- Classification of Ready-to-Eat Foods and regulatory limits
- Analyses to be performed according to classification:
  - Predictive microbiology
  - Durability studies or Shelf-life studies
  - Challenge Test
CHALLENGE TEST
- Principle
- Objectives of the Challenge Test
- Experimental design
  - Prerequisites
  - Test and control units
  - Selection of strains
  - Inoculation of test units
- Estimation of growth potential
- Estimation of growth kinetics parameters:
  - Lag phase duration
  - Maximum growth rate
- Test reports
CONCLUSIONS